English translation for "oligonucleotide probe"
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- 低核苷酸探测剂
Related Translations:
- Example Sentences:
| 1. | Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays
计算用于微阵列的基因表达谱、和基因分型研究技术平台使用的、低聚核苷酸探针装置的溶解温度分布。 | | 2. | 2 . the optimize of the oligonucleotide probes preparation the optimal condition is to resuspend the probes in ph = 9 , 0 . 1 m carbonate buffer to 100 m . 3
2 .寡核昔酸探针制备条件的优化以ph二9 ， 0 . im的碳酸盐缓冲液，重悬为100林m探针为最优条件。 | | 3. | Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end . then resuspended the lyophilized probes in ph = 9 , 0
寡核苷酸探针的制备根据genbank数据库新近公布的hla - dqa1等位基因序列，设计四组共16条特异性寡核苷酸分型探针，并在各5 ’端做氨基修饰。 | | 4. | In the present experiment studies , an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ) , cyclooxygenase - 2 ( cox - 2 ) , glial cell line - derived neurotrophic factor ( gdnf ) , caspase - 3 mrna and protein after brain injury in rats . immunocytochemical staining , western blotting , nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna , cox - 2 mrna , gdnf mrna , and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )
本实验从自行设计大鼠脑损伤落体打击器开始，先行建立了一个便于观察和施加处理因素、控制性好、重复性好的动物模型，选用30g击锤从25cm高处下落，冲击应力d为355 . 09kpa ，打击大鼠右顶部，造成中等程度的闭合性脑损伤，从病理形态学、组织超微结构观察及微管相关蛋白- 2 ( microtubuleassociatedprotein2 ， map - 2 ) 、环氧合酶- 2 ( cyclooxygenase2 ， cox - 2 ) 、胶质源性神经营养因子( glialcellline - derivedneutrophicfactor ， gdnf ) 、 caspase - 3基因及蛋白表达的时间性变化，详尽系统地阐述脑损伤后各指标变化的时间规律性及表达差异可能的形成机制。 | | 5. | The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide , and some factors affect immobile ratio of oligonucleotide probe , such as aminosilane treatment time , aldehyde treatment time , uv crosslinking energy , washing temperature and time
研究表明，氨基化试剂浓度对玻片荧光背景有影响，氨基化试剂处理时间、醛基化处理时间、紫外交联能量和洗涤温度和时间等工艺因素影响寡核苷酸探针的固定率。 | | 6. | The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area , which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis
而16srdna克隆文库分析和dna - dna杂交的结果表明“结核”区沉积物中这两类细菌数目很少，说明“暖池”区沉积物中的微生物群落结构特征是对环境因素的一种响应，同时也可能是影响该海区深海及海洋环境的一个重要因素。 | | 7. | Therefore , the determination of seb is very important for food hygienic analysis as well as for clinical analysis . nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology . the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule
其中b型葡萄球菌肠毒素( seb )是一种通常条件下更稳定，毒性最强的毒素，而核酸杂交技术则是分子生物学和基因工程中最常用和最基本的方法之一，因此本论文以该毒素的产毒基因为检测对象，以压电石英晶体为敏感元件，以合成的寡核苷酸探针为识别分子，构建了用于seb基因检测的压电生物传感器。 | | 8. | The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide . the more oligonucleotide probes have been linked with active group , the stronger fluorescence intensity is . for the strongest fluorescence , the technical conditions is : treatment of 2 % aminosilane of 20 minutes , treatment of 5 % aldehyde of 24 minutes , uv crosslinking of 150mj and washing of 5 minutes at 20
两种检测方法表明，当活性基团呈柱状、分布均匀且尺寸比较大( 200nm )时，有利于寡核苷酸探针的连接，且连接探针数量多，玻片表面荧光强度强，固定率高；当活性基团呈锥状、分布及尺寸不均匀( 150nm ( 300nm )时，连接的寡核苷酸探针数量少，玻片表面荧光强度弱，固定率低。 |
- Similar Words：
- "oligonucleotide arrays" English translation, "oligonucleotide hybridization" English translation, "oligonucleotide hybridization analysis" English translation, "oligonucleotide micro-chip" English translation, "oligonucleotide microarray" English translation, "oligonucleotide probe database" English translation, "oligonucleotide probe hybridization" English translation, "oligonucleotide synthesizer" English translation, "oligonucleotide-directed mutagenesis" English translation, "oligoocholia" English translation
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